我们的产品-蛋白快速提取试剂盒
返回

作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术（北京）有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品，其中具有多个特色产品，为蛋白提取分 离带来了新方案， 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中，产品 以其稳定的品质获得了世界各国越来越多的科研，药物开发及临床诊 断机构的青睐。



    订购信息：AT-022 / 4851.00元 (20 Preps)

    Minute™ 动物脂肪组织及细胞总蛋白提取试剂盒 AT-022

    Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes

    描述：

    脂肪组织特别是白色脂肪组织（WAT）已经被证实除了具有能量储存功能外，还与内分泌和器官炎症有关。从脂肪组织中提取和分析蛋白质对于了解许多生理/病理状态越来越重要。但是由于白色脂肪组织（WAT）和棕色脂肪组织（BAT）中高脂肪和低蛋白含量，所以在技术上极具挑战性。众所周知目前生物样品中水油乳化物最难分离，我公司研发的具有独特表面性质的带孔径的离心管柱和优化的无表面活性剂的缓冲液系统，可以快速有效的从脂肪组织匀浆中将水油乳化物分离。提取缓冲液比脂肪组织中的油冰点低，脂肪组织匀浆通过离心管柱能将水相和油相迅速分开，组织中的总蛋白无丢失。蛋白质得率可达2-3 mg/ml，远远高于其他方法。是世界上唯一一款脂肪蛋白提取商业试剂盒。

    应用：

    可应用于2-D、ELISA、SDS-PAGE、 immunoblottings、 IP、酶活性检测及其他应用。

    储存条件：

    Buffer A储存于4℃，其他部分15-30℃

    产品视频
    参考文献：AT-022文献列表

    • 1. Wang, N., Liu, Y., Ma, Y., & Wen, D. (2017). High-intensity interval versus moderate-intensity continuous training: Superior metabolic benefits in diet-induced obesity mice. Life Sciences.

      2. Qiantao Zheng(2017),Reconstitution of UCP1 using CRISPR/Cas9 in the white adipose tissue of pigs decreases fat deposition and improves thermogenic capacity. www.pnas.org/cgi/doi/10.1073/pnas.1707853114
      3. Li etal (2017).High Efficiency Protein Extraction from Adipose Tissues3. BioTechniques 63:191 (November 2017) DOI: 10.2144/000114601
      4. Micah L. Battson, Dustin M Lee, Dillon K Jarrell, Shuofei Hou, Kayl E Ecton, Tiffany L Weir, and Christopher L Gentile（2017）.Suppression of Gut Dysbiosis Reverses Western Diet-Induced Vascular Dysfunction. AM J PHYSIOL-ENDOC M doi.org/10.1152/ajpendo.00187.2017
      5. Guizong Chen, Qin Li ,Yanping Luo, Tao Liu, Shaolong ,Zhou Er Pan, Lixia Peng(2018).Effect of Notoginsenoside R1 on autologous adipose graft in rats.spandidos-publications.com. doi.org/10.3892/mmr.2018.8596
      6. Tianxing Chen,Xiaoying Cheng,Yun Wang,Wu Yin（2018）.Toosendanin inhibits adipogenesis by activating Wnt/β-catenin signaling.SCiENtifiC REPorTS. DOI:10.1038/s41598-018-22873-x
      7. N Wang ,Y Liu ,Y Ma ,D Wen (2018).Hydroxytyrosol ameliorates insulin resistance by modulating endoplasmic reticulum stress and prevents hepatic steatosis in diet-induced obesity mice. Journal of Nutritional Biochemistry , doi.org/10.1016/j.jnutbio.2018.03.018
      8. Weijie Du, Arnold Piek, E. Marloes Schouten, Cees W.A. van de Kolk, Christian Mueller, Alexandre Mebazaa, Adriaan A. Voors, Rudolf A. de Boer,Herman H.W. Sillje(2018).Plasma levels of heart failure biomarkers are primarily a reflection of extracardiac production.Theranostics . doi: 10.7150/thno.26055
      9. Melissa Hann(2018).Anti-inflammatory Activity of Isomaltodextrin in a C57BL/6NCrl Mouse Model with Lipopolysaccharide-induced Low-grade Chronic Inflammation. http://hdl.handle.net/10214/14124.
      10. Raffael Ott etal (2018). Reduced insulin receptor expression and altered DNA methylation in fat tissues and blood of women with GDM and offspring.The Journal of Clinical Endocrinology & Metabolism, jc.2018-01659, doi.org/10.1210/jc.2018-01659.
      11. Lei Liu, Ningning Wang etal (2018).Saponins from Boussingaultia gracilis prevent obesity and related metabolic impairments in diet-induced obese mice. Food Funct. DOI: 10.1039/C8FO01264D.
      12. SJ Yue (2018).Berberine alleviates insulin resistance by reducing peripheral branched-chain amino acids.Am J Physiol Endocrinol Metab. 2018 Nov 13. doi: 10.1152/ajpendo.00256.2018.
      13. Shah, A. P., Johnson, M. D., Fu, X., Boersma, G. J., Shah, M., Wolfgang, M. J., ... & Baraban, J. M. (2019). Deletion of translin (Tsn) induces robust adiposity and hepatic steatosis without impairing glucose tolerance. International Journal of Obesity.doi.org/10.1038/s41366-018-0315-7.
      14. Li M et al(2019). TNF-α Upregulates IKKε Expression via the Lin28B/let-7a Pathway to Induce Catecholamine Resistance in Adipocytes.Obesity (Silver Spring). Doi: 10.1002/oby.22434.

      15. Jahandideh, F., de Campos Zani, S. C., Son, M., Proctor, S. D., Davidge, S. T., Chan, C. B., & Wu, J. (2019). Egg white hydrolysate enhances insulin sensitivity in high fat diet induced insulin resistant rats via AKT activation. British Journal of Nutrition, 1-25. DOI10.1017/S0007114519000837.
      16. Zhang, F., Li, D., Wu, Q., Sun, J., Guan, W., Hou, Y., ... & Wang, J. (2019). Prepartum body conditions affect insulin signaling pathways in postpartum adipose tissues in transition dairy cows. Journal of Animal Science and Biotechnology, 10(1), 38.doi.org/10.1186/s40104-019-0347-4
      17. Wang, N., Ma, Y., Liu, Z., Liu, L., Yang, K., Wei, Y., ... & Wen, D. (2019). Hydroxytyrosol prevents PM2. 5-induced adiposity and insulin resistance by restraining oxidative stress related NF-κB pathway and modulation of gut microbiota in a murine model. Free Radical Biology and Medicine, 141, 393-407. Zani, S. (2019).

      18. Campos Zani, S. C（2019）.Exploratory study of egg white hydrolysate mechanisms of action in insulin sensitive tissues of high fat diet-induced insulin resistant rats. doi.org/10.7939/r3-vpcv-w240
      19. Vianello, E., Dozio, E., Bandera, F., Froldi, M., Micaglio, E., Lamont, J., ... & Schmitz, G. (2020). Correlative Study on Impaired Prostaglandin E2 Regulation in Epicardial Adipose Tissue and its Role in Maladaptive Cardiac Remodeling via EPAC2 and ST2 Signaling in Overweight Cardiovascular Disease Subjects. International Journal of Molecular Sciences, 21(2), 520.

      20. Fu, X., Shah, A. P., Li, Z., Li, M., Tamashiro, K. L., & Baraban, J. M. (2020). Genetic inactivation of the translin/trax microRNA-degrading enzyme phenocopies the robust adiposity induced by Translin (Tsn) deletion. bioRxiv

      21. An, Y. A. and Scherer, P. E. (2020). Mouse Adipose Tissue Protein Extraction. Bio-protocol 10(11): e3631. DOI: 10.21769/BioProtoc.3631.

      22. Tu, J., Zhu, S., Li, B., Xu, G., Luo, X., Jiang, L., ... & Chen, C. (2020). Gegen Qinlian Decoction Coordinately Regulates PPARγ and PPARα to Improve Glucose and Lipid Homeostasis in Diabetic Rats and Insulin Resistance 3T3-L1 Adipocytes. Frontiers in Pharmacology, 11.

      23. Oldoni, F., Cheng, H., Banfi, S., Gusarova, V., Cohen, J. C., & Hobbs, H. H. (2020). ANGPTL8 has both endocrine and autocrine effects on substrate utilization. JCI insight.

      24.Li, X., Lu, H. Y., Jiang, X. W., Yang, Y., Xing, B., Yao, D., ... & Zhao, Q. C. (2020).          Cinnamomum cassia extract promotes thermogenesis during exposure to cold via activation of brown adipose tissue. Journal of Ethnopharmacology, 113413.

      25.Jiang, X. W., Lu, H. Y., Xu, Z. H., Zhang, Y. S., & Zhao, Q. C. (2020). Network pharmacology-based research uncovers cold resistance and thermogenesis mechanism of Cinnamomum cassia. Fitoterapia, 104824.

      26.Cheng, C., Xue, F., Sui, W., Meng, L., Xie, L., Zhang, C., ... & Zhang, Y. (2021). Deletion of natriuretic peptide receptor C alleviates adipose tissue inflammation in hypercholesterolemic Apolipoprotein E knockout mice. Journal of Cellular and Molecular Medicine.

      27.Li, Y., Wang, H., Liao, Y., Yan, Q., Ouyang, Z., Zhao, Y., ... & Lai, L. (2021). CREBRF p. Arg457Gln Promotes Obesity and Improves Insulin Sensitivity by Promoting Preadipocyte Differentiation and Reducing Oxidative Metabolism in Gene-modified Pig Models.

      28.Wang, Y., Jiang, H. F., Liu, B. B., Chen, L. L., Wang, Y., Liu, X. Y., ... & Wu, X. F. (2021). Brown Adipose Tissue Activation Is Involved in Atherosclerosis of ApoE−/− Mice Induced by Chronic Intermittent Hypoxia. Frontiers in Cardiovascular Medicine, 8.

      29.Hill, J. L., McIver, K. B., Katzer, K., & Foster, M. T. (2022). Capillary Western Immunoassay Optimization of Estrogen Related Factors in Human Subcutaneous Adipose Tissue. Methods and Protocols, 5(2), 34.

      30.Liu, Z., Chen, T., Zhang, S., Yang, T., Gong, Y., Deng, H. W., ... & Chen, Y. (2022). Discovery and functional assessment of a novel adipocyte population driven by intracellular Wnt/β-catenin signaling in mammals. eLife, 11, e77740.

      31.de Campos Zani, S. C., Son, M., Bhullar, K. S., Chan, C. B., & Wu, J. (2022). IRW (Isoleucine–Arginine–Tryptophan) Improves Glucose Tolerance in High Fat Diet Fed C57BL/6 Mice via Activation of Insulin Signaling and AMPK Pathways in Skeletal Muscle. Biomedicines, 10(6), 1235.

      32.Cao, S., Pan, Y., Tang, J., Terker, A. S., Arroyo Ornelas, J. P., Jin, G. N., ... & Zhang, M. Z. (2022). EGFR-mediated activation of adipose tissue macrophages promotes obesity and insulin resistance. Nature Communications, 13(1), 1-16.

      33.GnRH receptor mediates lipid storage in female adipocytes via AMPK pathway. Int J Med Sci, 19(9), 1442-1450.

      34.Kristina Schlicht; Juliane Brandes; Isabelle Zobel; Nathalie Rohmann; Corinna Geisler; et al（2022）.Dipeptidylpeptidase (DPP)-4 inhibitor therapy increases circulating levels of anti-inflammatory soluble frizzle receptor protein (sFRP)-5 which is decreased in severe COVID-19 disease.Diabetologie Und Stoffwechsel

      35.de Campos Zani, S. C., Wang, R., Veida-Silva, H., Clugston, R. D., Yue, J. T., Mori, M. A., ... & Chan, C. B. (2023). An Egg White-Derived Peptide Enhances Systemic Insulin Sensitivity and Modulates Markers of Non-Alcoholic Fatty Liver Disease in Obese, Insulin Resistant Mice. Metabolites, 13(2), 174.

      36.Li, D., Chen, B., Yuan, S., Zhang, M., Wang, X., Liu, R., ... & Chen, X. (2023). Analysis of Adipose Tissue Cytokine Profile in a Chronic Kidney Disease Model with Insulin Resistance.

      37.Hunstiger, M., Johannsen, M. M., & Oliver, S. R. (2023). Non-shivering thermogenesis is differentially regulated during the hibernation season in Arctic ground squirrels. Frontiers in Physiology, 14.

      38.Cheng, C., Zhang, J., Li, X., Xue, F., Cao, L., Meng, L., ... & Zhang, C. (2023). NPRC deletion mitigated atherosclerosis by inhibiting oxidative stress, inflammation and apoptosis in ApoE knockout mice. Signal Transduction and Targeted Therapy, 8(1), 290.

      39.Gehle, P. F., Satzinger, S., Willenborg, S., & Eming, S. A. (2023). Isolation of dermal adipocytes from mouse skin for biochemical analysis. STAR protocols, 4(4), 102765.

      40.de Campos Zani, S. C., Berg, E., Jiang, X., Knox, A., Ackroyd, E., Getachew, A., ... & Chan, C. B. (2024). IRW improves diet-induced non-alcoholic fatty liver disease by reducing steatosis associated with increased capacity for oxidative phosphorylation. Journal of Functional Foods, 112, 105976.

      41.Lu, M., Yu, Z., Yang, X., An, L., Jing, X., Xu, T., ... & Yu, Z. (2024). Remodelling the inguinal adipose sensory system to switch on the furnace: Electroacupuncture stimulation induces brown adipose thermogenesis. Diabetes, Obesity and Metabolism.

      42.Jia, W., Giwa, R., Moley, J. R., Smith, G. I., Petersen, M. C., Field, R. L., ... & Brestoff, J. R. (2024). MXRA8 promotes adipose tissue whitening to drive obesity. bioRxiv, 2024-01.

      43.Smagris, E., Shihanian, L. M., Mintah, I. J., Bigdelou, P., Livson, Y., Brown, H., ... & Gusarova, V. (2024). Divergent role of Mitochondrial Amidoxime Reducing Component 1 (MARC1) in human and mouse. PLoS genetics, 20(3), e1011179.





    close
    close