作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术(北京)有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品,其中具有多个特色产品,为蛋白提取分 离带来了新方案, 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中,产品 以其稳定的品质获得了世界各国越来越多的科研,药物开发及临床诊 断机构的青睐。



    订购信息:WA-009 / 530.00元(10ml)   WA-009 / 60.00元(1ml)

    Minute™ 变性蛋白溶解液 WA-009

    Minute™ Denaturing Protein Solubilization Reagent

    描述:

    此试剂用于溶解Invent试剂盒提取(目录号:SM-005,SM-005-P,MP-007,CP-011,NE-013,YM-017,MM-018和AF-023,ER-035,ER-036等)或其他公司试剂盒提取的不可溶性蛋白。溶解的蛋白质在阴离子表面活性剂(SDS)作用下成为变性蛋白,本试剂溶解的蛋白质主要应用于SDS-PAGE电泳和WB。一般情况下,溶解的蛋白质使用SDS去除剂(货号WA-012,Invent)去除SDS后也可用于免疫沉淀和胰酶消化。

    储存条件:

    常温

    参考文献:WA-009文献列表
    • 1. Xiaoqing Xu, Jia Xu, Jiacheng Wu, Ye Hu, Yanmei Han, Yan Gu, Kai Zhao, Qian Zhang, Xingguang Liu, Juan Liu, Bing Liu, and Xuetao Cao (2018).Phosphorylation-Mediated IFN-γR2 Membrane Translocation Is Required to Activate Macrophage Innate Response.cell.doi.org/10.1016/j.cell.2018.09.011.
      2. Peng, W., Tong, C., Li, L., Huang, C., Ran, Y., Chen, X., ... & Luo, X. (2019). Trophoblastic proliferation and invasion regulated by ACTN4 is impaired in early onset preeclampsia. The FASEB Journal, fj-201802058RR.Doi: 10.1096/fj.201802058RR.

      3. Ghosh, M., Lo, R., Ivic, I., Aguilera, B., Qendro, V., Devarakonda, C., & Shapiro, L. H. (2019). CD13 tethers the IQGAP1-ARF6-EFA6 complex to the plasma membrane to promote ARF6 activation, β1 integrin recycling, and cell migration. Signal., 12(579), eaav5938.DOI: 10.1126/scisignal.aav5938.

      4.Li, Y., Tang, W., Kang, L., Kong, S., Dong, Z., Zhao, D., ... & Yu, S. (2021). Functional correlation of ATP1A2 mutations with phenotypic spectrum: from pure hemiplegic migraine to its variant forms. The Journal of Headache and Pain, 22(1), 1-12.

      5.Weishu Ren, Haoyang Lou, Xinghua Ren, Gehua Wen, Xue Wu, Xi Xia, Shuying Wang, Xiaojin Yu, Lei Yan, Guohua Zhang, Jun Yao, Yan Lu, Xu Wu,Ketamine promotes the amyloidogenic pathway by regulating endosomal pH,Toxicology,Volume 471,2022

      6.44.Weikai Hu, Yinan Guo, Xueyan Wang, Tianyu Cui, Chunyun Li, Ruixia Liu, Chenghong Yin.(2022).Angiotensin-(1–7) promotes mitochondrial translocation of human telomerase reverse transcriptase in HUVECs through the TOM20 complex,Archives of Biochemistry and Biophysics,Volume 722,2022




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