作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术(北京)有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品,其中具有多个特色产品,为蛋白提取分 离带来了新方案, 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中,产品 以其稳定的品质获得了世界各国越来越多的科研,药物开发及临床诊 断机构的青睐。



    订购信息:NT-032 / 3376.00元(20 tests)

    Minute™新鲜/冷冻组织胞质胞核分离试剂盒 NT-032

    Minute™ Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues

    描述:

    从细胞和组织中分离细胞质和细胞核组分是一种常见的实验。细胞样品的组分分离相对简单直接,然而,从组织样品中特别是冷冻组织中将胞质和胞核组分分离干净是很具挑战的。因为冷冻和反复冻融会引起组织细胞结构的改变,加之不适当的匀浆方式和效率低的提取缓冲液,使得交叉污染十分严重。目前大多数商业试剂盒设计可同时用于细胞和组织样品,但是忽略了两种类型样品之间的结构差异。因此,从新鲜/冷冻组织中分离胞质和胞核组分时,交叉污染问题无法避免(见下文参考文献1和2)。这款新型的胞质和胞核分离试剂盒专用于组织样品的胞质胞核分离,试剂盒特有的缓冲液体系和独特的操作方法可以将交叉污染降到最低。整个操作可以在30分钟内完成。

    应用:

    可应用于SDS-PAGE,immunoblottings,ELISA,IP,蛋白定位,凝胶迁移分析,2-D等其他应用。

    储存:

    4℃

    参考文献:NT-032文献列表
    • 1.Li, Y., Wu, J., Yu, S., Zhu, J., Zhou, Y., Wang, P., ... & Zhao, Y. (2020). Sestrin2 promotes angiogenesis to alleviate brain injury by activating Nrf2 through regulating the interaction between p62 and Keap1 following photothrombotic stroke in rats.Brain Research, 146948.

      2.Wang, B., Huang, X., Pan, X., Zhang, T., Hou, C., Su, W. J., ... & Wang, Y. X. (2020). Minocycline prevents the depressive-like behavior through inhibiting the release of HMGB1 from microglia and neurons.Brain, Behavior, and Immunity.

      3.Song T, Guan X, Wang X,et al. Dynamic modulation of gut microbiota improves post-myocardial infarct tissue repair in rats via butyric acid-mediated histone deacetylase inhibition.The FASEB Journal. 2021;35:e21385.

      4.Zhou, J., Zhao, Y., Guo, Y. J., Zhao, Y. S., Liu, H., Ren, J., ... & Ji, E. S. (2021). A rapid juvenile murine model of nonalcoholic steatohepatitis (NASH): Chronic intermittent hypoxia exacerbates Western diet-induced NASH. Life Sciences, 119403.

      5.Bao, J., Lu, Y., She, Q. Y., Dou, W., Tang, R., Xu, X., ... & Zheng, C. (2021). MicroRNA-30 regulates left ventricular hypertrophy in chronic kidney disease. JCI insight.

      6.Zhou, Y., Nie, A. Q., Chen, S., Wang, M. M., Yin, R., Tang, B. H., ... & Zhao, W. (2021). Downregulation of Renal MRPs Transporters in Acute Lymphoblastic Leukemia Mediated by the IL-6/STAT3/PXR Signaling Pathway. Journal of Inflammation Research, 14, 2239-2252.

      7.Yu, D., Zarate, N., Cuccu, F., Yue, J. S., Brown, T. G., Nanclares, T. N. M., ... & Gomez-Pastor, R. (2021). CK2 alpha prime and alpha-synuclein pathogenic functional interaction mediates inflammation and transcriptional dysregulation in Huntington’s disease 2. neurodegenerative diseases, 70, 71.

      8.Weng, J., Wang, X., Xu, B., & Li, W. (2021). Augmenter of liver regeneration ameliorates ischemia-reperfusion injury in steatotic liver via inhibition of the TLR4/NF-κB pathway. Experimental and Therapeutic Medicine, 22(2), 1-8

      9.Wang, M., Wang, L., Zhou, Y., Feng, X., Ye, C., & Wang, C. (2021). Icariin attenuates renal fibrosis in chronic kidney disease by inhibiting interleukin-1β/transforming growth factor-β-mediated activation of renal fibroblasts. Phytotherapy Research, 1–12.

      10.Yang, C., Luo, P., Chen, S. J., Deng, Z. C., Fu, X. L., Xu, D. N., ... & Liu, W. J. (2021). Resveratrol sustains intestinal barrier integrity, improves antioxidant capacity, and alleviates inflammation in the jejunum of ducks exposed to acute heat stress. Poultry Science, 101459.

      11.Imai, S., Ohama, M., Suzuki, M., Katayanagi, Y., Kayashima, Y., & Tezuka, H. Short Alkyl Chain-Length Resorcinol Olivetol Protects Against Obesity with Mitochondrial Activation by Pgc-1α Deacetylation. Available at SSRN 3924601.





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