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作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术（北京）有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品，其中具有多个特色产品，为蛋白提取分 离带来了新方案， 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中，产品 以其稳定的品质获得了世界各国越来越多的科研，药物开发及临床诊 断机构的青睐。



    订购信息：SA-02-BT / 4263.00元(50 Preps)

    Minute™ 骨组织总蛋白提取试剂盒 SA-02-BT

    Minute™ Total Protein Extraction Kit for Bone Tissue

    描述：

    骨组织通常用于研究的有两种类型：密质骨和松质骨。骨组织中的细胞紧密堆积，由于其独特的结构，很难有效地从中提取总蛋白质。使用传统的溶液法提取蛋白质如RIPA效率低，产率也很低，提取的蛋白质图谱也是不完整的。本试剂盒利用离心管技术以及优化的裂解液，利用物理研磨和化学裂解相结合，提供了一种简单和快速的从人或动物骨组织中提取蛋白质的高效方法。可根据特定的下游应用选择变性裂解液或天然裂解液。整个过程不到10分钟即可完成，蛋白质得率根据骨骼样品类型不同在0.5-2mg/ml范围。试剂盒所提供试剂及耗材可以满足50个样品实验。


    应用：

    SDS-PAGE, WB，IP，ELISA，酶活性测定和蛋白质组学分析。缓冲剂与IMAC树脂兼容，可用于His标签蛋白纯化。在质谱分析之前需将提取的蛋白质样品中的盐和表面活性剂除去。


    储存条件：

    RT

    产品视频
    参考文献：SA-02-BT文献列表

    • 1. Ren, H., Wang, Z., Xu, J., Chen, J., & Lan, J. (2020). The Impact of Frizzled-9 on Dental Implant Osseointegration in Hyperlipidemic Rats. Journal of Hard Tissue Biology, 29(1), 37-44.

      2. Pedersen, K. B., Osborn, M. L., Robertson, A. C., Williams, A. E., Watt, J., Denys, A., ... & Ronis, M. J. (2020). Chronic Ethanol Feeding in Mice Decreases Expression of Genes for Major Structural Bone Proteins in a Nox4-Independent Manner. Journal of Pharmacology and Experimental Therapeutics, 373(3), 337-346.

      3.He-dong Liu, Mao-xian Ren, Yang Li, Ruo-tian Zhang, Neng-feng Ma, Tianlin Li, Wen-kai Jiang, Zhi Zhou, Xue-wei Yao, Zhi-yi Liu & Min Yang (2022): Melatonin alleviates hydrogen peroxide induced oxidative damage in MC3T3-E1 cells and promotes osteogenesis by activating SIRT1, Free Radical Research, DOI: 10.1080/10715762.2022.2037580

      4.Chaolai Jiang; Yiwei Lin; Haojie Shan; Wenyang Xia; Chenhao Pan; et al(2022).miR-146a Protects against Staphylococcus aureus-Induced Osteomyelitis by Regulating Inflammation and Osteogenesis.ACS Infectious Diseases.

      5.Song, D., Ha, C. Z., Xu, Q., & Hu, Y. H. (2022). mRNA expression profiling of the cancellous bone in patients with idiopathic osteonecrosis of the femoral head by whole-transcriptome sequencing. Medicine, 101(35), e30213.

      6.Deng, J., Cohen, D. J., Sabalewski, E. L., Van Duyn, C., Wilson, D. S., Schwartz, Z., & Boyan, B. D. (2022). Semaphorin 3A Delivered by a Rapidly Polymerizing Click Hydrogel Overcomes Impaired Implant Osseointegration in a Rat Type 2 Diabetes Model. Acta Biomaterialia.

      7.He, H., Zhang, Y., Sun, Y., Zhang, Y., Xu, J., Yang, Y., & Chen, J. (2022). Folic Acid Attenuates High-Fat Diet-Induced Osteoporosis Through the AMPK Signaling Pathway. Frontiers in cell and developmental biology, 9, 3661.

      8.Choi, R. Y., Kim, I. W., Ji, M., Paik, M. J., Ban, E. J., Lee, J. H., ... & Seo, M. (2023). Protaetia brevitarsis seulensis larvae ethanol extract inhibits RANKL-stimulated osteoclastogenesis and ameliorates bone loss in ovariectomized mice. Biomedicine & Pharmacotherapy, 165, 115112.

      9.Liu, Z., Yao, X., Jiang, W., Zhou, Z., & Yang, M. (2023). Sodium butyrate enhances titanium nail osseointegration in ovariectomized rats by inhibiting the PKCα/NOX4/ROS/NF-κB pathways. Journal of Orthopaedic Surgery and Research, 18(1), 1-14.

      10.Zhang, C. X., & Man, Q. W. (2024). Proteomics study of bone tissue around ameloblastoma and the potential mechanism of CD36 in bone remodelling. British Journal of Oral and Maxillofacial Surgery.





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