作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术(北京)有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品,其中具有多个特色产品,为蛋白提取分 离带来了新方案, 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中,产品 以其稳定的品质获得了世界各国越来越多的科研,药物开发及临床诊 断机构的青睐。



    订购信息:AT-022 / 3810.00元 (20 Tests)

    Minute™ 动物脂肪组织及细胞总蛋白提取试剂盒 AT-022

    Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes

    描述:

    脂肪组织特别是白色脂肪组织(WAT)已经被证实除了具有能量储存功能外,还与内分泌和器官炎症有关。从脂肪组织中提取和分析蛋白质对于了解许多生理/病理状态越来越重要。但是由于白色脂肪组织(WAT)和棕色脂肪组织(BAT)中高脂肪和低蛋白含量,所以在技术上极具挑战性。众所周知目前生物样品中水油乳化物最难分离,我公司研发的具有独特表面性质的带孔径的离心管柱和优化的无表面活性剂的缓冲液系统,可以快速有效的从脂肪组织匀浆中将水油乳化物分离。提取缓冲液比脂肪组织中的油冰点低,脂肪组织匀浆通过离心管柱能将水相和油相迅速分开,组织中的总蛋白无丢失。蛋白质得率可达2-3 mg/ml,远远高于其他方法。是世界上唯一一款脂肪蛋白提取商业试剂盒。

    应用:

    可应用于2-D、ELISA、SDS-PAGE、 immunoblottings、 IP、酶活性检测及其他应用。

    储存条件:

    Buffer A储存于4℃,其他部分15-30℃

    参考文献:AT-022文献列表
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