作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术(北京)有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品,其中具有多个特色产品,为蛋白提取分 离带来了新方案, 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中,产品 以其稳定的品质获得了世界各国越来越多的科研,药物开发及临床诊 断机构的青睐。



    订购信息:YT-015 / 2678.00元 (50 Tests)

    Minute™ 酵母细菌微藻等厚胞壁微生物总蛋白提取试剂盒 YT-015

    Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls

    描述:

    Minute厚壁微生物总蛋白提取试剂盒无需仪器,可以从酵母,丝状真菌,革兰氏阳性和阴性菌虫卵,微藻中等提取总蛋白。试剂盒同时提供优化的变性裂解液和天然裂解液可供不同下游实验选择。与传统方法比无需复杂的实验条件,从酵母中提取某些分子量范围的蛋白质更优于其他方法。可用于对数期和静止期微生物。试剂盒所有步骤在单一管内完成,时间<10分钟,得率可达2-5 mg/ml。


     应用:

    提取的蛋白质样品可以应用于SDS-PAGE, WB,IP, ELISA,酶活性检测和蛋白质组学分析。缓冲液可以兼容IMAC树脂用于His-标签蛋白纯化。


    储存条件:

    室温

    参考文献:YT-015文献列表
    • 1. Nagayoshi, Y., Miyazaki, T., Shimamura, S., Nakayama, H., Minematsu, A., Yamauchi, S., ... & Mukae, H. (2017). Unexpected effects of azole transporter inhibitors on antifungal susceptibility in Candida glabrata and other pathogenic Candida species. PloS one, 12(7), e0180990.
      2. Ahmad Al-Omari etal (2018).Discovering regulators in Post-Transcriptional control of the Biological Clock of Neurospora crassa using Variable Topology Ensemble Methods on GPUs. IEEE Access. DOI: 10.1109/ACCESS.2018.2871876.
      3. Mia Terashima, et al (2018).Antarctic heterotrophic bacterium Hymenobacter nivis P3T displays light-enhanced growth and expresses putative photoactive proteins.Environ Microbiol Rep.doi: 10.1111/1758-2229.12702.
      4. W Lin et al (2018).The cellular and molecular mechanism of glutaraldehyde-didecyldimethylammonium bromide as a disinfectant against Candida albicans. Applied Microbiology.doi: 10.1111/jam.14142.
      5. Shimamura, S., Miyazaki, T., Tashiro, M., Takazono, T., Saijo, T., Yamamoto, K., ... & Mukae, H. (2019). Autophagy-Inducing Factor Atg1 Is Required for Virulence in the Pathogenic Fungus Candida glabrata. Frontiers in microbiology, 10.

      6. Wang, Y., Ma, Y., Wang, D., Liu, W., Chen, J., Jiang, Y., ... & Qin, L. (2019). Polar tube structure and three polar tube proteins identified from Nosema pernyi. Journal of Invertebrate Pathology, 107272.

      7.Li Wang, Jing Liu, Monika Filipiak, Khongorzul Mungunkhuyag, Paweł Jedynak, Jan Burczyk, Pengcheng Fu, Przemysław Malec,Fast and efficient cadmium biosorption by Chlorella vulgaris K-01 strain: The role of cell walls in metal sequestration,
      Algal Research,Volume 60,2021




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