作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术(北京)有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品,其中具有多个特色产品,为蛋白提取分 离带来了新方案, 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中,产品 以其稳定的品质获得了世界各国越来越多的科研,药物开发及临床诊 断机构的青睐。



    订购信息:SM-005-P / 5699.00元(50tests)

    Minute™ 植物膜蛋白提取试剂盒 SM-005-P

    Minute™ Plasma Membrane Protein Isolation Kit for Plants

    描述:

    植物膜蛋白占植物细胞总蛋白的很小一部分,但是在植物生理学中起着非常重要的作用。传统的植物膜蛋白分离纯化方法是蔗糖密度梯度离心法和双液相法。这些方法虽然比较有效,但是需要超高速离心和大量的起始原材料,操作过程十分繁琐和费时。本试剂盒解决了植物膜蛋白提取中的难点。植物组织首先通过缓冲液 A 中致敏,匀浆,然后通过离心管柱,在此过程中匀浆的组织通过柱子特有的 Z 字形通路后细胞膜被切割成大小相等的碎片,后续无需超高速离心,通过差速离心和密度离心可将天然的质膜蛋白从未破裂的细胞,细胞核,细胞质和细胞器的混合物中分离出来。在每次实验中仅需使用相同量的起始材料,离心力和离心时间,即可高度富集膜蛋白,并保证一致性良好。整个操作过程大约 1 小时可以完成。
    应用:

    试剂盒用于快速从植物组织中分离天然膜蛋白,可应用于 SDS-PAGE,immunoblottings,ELISA,IP,膜蛋白质结构分析,2-D,酶活性测定及其他应用。
    储存条件:

    -20℃储存

    参考文献:SM-005-P文献列表
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