作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术(北京)有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品,其中具有多个特色产品,为蛋白提取分 离带来了新方案, 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中,产品 以其稳定的品质获得了世界各国越来越多的科研,药物开发及临床诊 断机构的青睐。



    订购信息:CP-011 / 3306.00元 (50Tests)   CP-011 / 346.00元 (4Tests)

    Minute™ 植物叶绿体分离试剂盒 CP-011

    Minute™ Chloroplast Isolation Kit

    描述:

    叶绿体分离试剂盒中包含优化的叶绿体提取缓冲液和2.0ml离心管柱。利用带孔径的离心管柱技术可以快速从新鲜的植物组织中(叶片,种子,软茎等)提取完整的叶绿体。其他方法都需要1-10g组织来提取叶绿体,我们的试剂盒可以从100-200mg新鲜植物叶片中快速提取1 X 106 - 1 X 107 的完整叶绿体(>90%完整),操作时间<5分钟。
    应用:

    提取出的叶绿体可以应用于生化分析,SDS-PAGE,immunoblottings,叶绿体酶测定等。叶绿体也可作为RNA和DNA纯化起始材料用于分子生物学研究。
    特点:

    1、简单,快速,性价比高;

    2、得率高;

    3、小样品量,唯一可从小样本中提取叶绿体的商业试剂盒;

    4、适用于筛选或下游应用(如叶绿体DNA提取)。
    储存条件:

    储存于-20℃

    参考文献:CP-011文献列表
    • 1. H Oh et al. (2015) Two complete chloroplast genome sequences of Cannabis sativa varieties Mitochondrial DNA. DOI:10.3109/19401736.2015.1053117.
      2. Hu, D., Li, Y., Jin, W., Gong, H., He, Q., & Li, Y. (2017). Identification and Characterization of a Plastidic Adenine Nucleotide Uniporter (OsBT1-3) Required for Chloroplast Development in the Early Leaf Stage of Rice. Scientific RepoRts | 7:41355 | DOI: 10.1038/srep41355.
      3. Kanazawa, A., Ostendorf, E., Kohzuma, K., Hoh, D., Strand, D. D., Sato-Cruz, M., ... & Kramer, D. M. (2017).Chloroplast ATP Synthase Modulation of the Thylakoid Proton Motive Force:Implications for Photosystem I and Photosystem II Photoprotection. Front. Plant Sci., 03 May 2017 | DOI: org/10.3389/fpls.2017.00719
      4. Suzuki, J.Y., Geib, S.M., Carlsen, M.M., Henriquez, C.L., Amore, T.D., Sim, S.B., Matsumoto, T.K., Keith, L.M. and Myers, R.Y. (2017). Development of chloroplast single nucleotide polymorphisms (SNPs) as a tool towards interspecies typing of Anthurium germplasm. Acta Hortic. 1167, 257-270
      5. H ShuoHao etal (2018).Identification and characterization of a pyridoxal 5′-phosphate phosphatase in tobacco plants. Plant science.doi.org/10.1016/j.gene.2018.10.078.
      6. Fuli Ma etal (2019).Dynamic translocation of stilbene synthase VpSTS29 from a Chinese wild Vitis species upon UV irradiation.Phytochemistry.doi.org/10.1016/j.phytochem.2018.12.019.
      7. Ren, R. C., Wang, L. L., Zhang, L., Zhao, Y. J., Wu, J. W., Wei, Y. M., ... & Zhao, X. Y. DEK43 is a P‐type PPR Protein Responsible for the Cis‐splicing of nad4 in Maize Mitochondria. Journal of Integrative Plant Biology.doi: 10.1111/jipb.12843.

      8. Lee, S., Joung, Y. H., Kim, J. K., Do Choi, Y., & Jang, G. (2019). An isoform of the plastid RNA polymerase-associated protein FSD3 negatively regulates chloroplast development. BMC Plant Biology, 19(1), 524.
      9. Yin, B., Zhang, J., Liu, Y., Pan, X., Zhao, Z., Li, H., ... & Liu, D. (2019). PtomtAPX, a mitochondrial ascorbate peroxidase, plays an important role in maintaining the redox balance of Populus tomentosa Carr. Scientific Reports, 9(1), 1-15.
      10. Wang chengliang et al(2020).Chloroplastic Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice.NEW PHYTOLOGIST.
      11. Shoeva, O. Y., Mursalimov, S. R., Gracheva, N. V., Glagoleva, A. Y., Börner, A., & Khlestkina, E. K. (2020). Melanin formation in barley grain occurs within plastids of pericarp and husk cells. Scientific Reports, 10(1), 1-9

      12.Long, J., Xing, W., Wang, Y., Wu, Z., Li, W., Zou, Y., ... & Zhang, F. (2021). Comparative Proteomic Analysis on Chloroplast Proteins Provides New Insights Into the Effects of Low Temperature in Sugar Beet.

      13.Shapiro, K. O. (2021). Plug and play: Is “directed endosymbiosis” of chloroplasts possible?. bioRxiv.

      14.Pan, T., Liu, Y., Ling, C., Tang, Y., Tang, W., Liu, Y., ... & Wang, S. (2021). CV-containing vesicle budding from chloroplast inner envelope membrane is mediated by clathrin but inhibited by GAPC. bioRxiv.

      15.Li, C., Li, J., Du, X., Zhang, J., Zou, Y., Liu, Y., ... & Lu, H. (2022). Chloroplast Thylakoidal Ascorbate Peroxidase, PtotAPX, Has Enhanced Resistance to Oxidative Stress in Populus tomentosa. International Journal of Molecular Sciences, 23(6), 3340.





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