作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术(北京)有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品,其中具有多个特色产品,为蛋白提取分 离带来了新方案, 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中,产品 以其稳定的品质获得了世界各国越来越多的科研,药物开发及临床诊 断机构的青睐。



    订购信息:ED-028 / 5995.00元 (20 tests)

    Minute™ 内体和细胞组分分离试剂盒 ED-028

    Minute™ Endosome Isolation and Cell Fractionation Kit

    描述:
    初级内体(EE)是次级成熟内体的起点,初级内体主要是由内吞的囊泡相互融合形成。初级内体不仅仅通过网格蛋白介导的信号通路接受胞吞物,还有其他许多通路。胞吞机制除了在维持正常细胞生理中起到重要作用,还在阿尔兹海默病和遗传性溶酶体储积症等许多疾病中发挥了很大作用。

    应用:

    本试剂盒可以从培养的细胞和组织样品中大量沉淀富集初级内体,用于SDS-PAGE、immunoblottings、ELISA、IP、MS、2-D、酶活性检测等其他应用。

    特点:

    1、简单,快速,性价比高;

    2、无表面活性剂及EDTA,天然细胞组份分离(细胞核,细胞质, 细胞器和质膜,内体);

    3、小样品量

    储存条件:

    储存于4℃

    参考文献:ED-028文献列表
    • 1. Xiaoqing Xu, Jia Xu, Jiacheng Wu, Ye Hu, Yanmei Han, Yan Gu, Kai Zhao, Qian Zhang, Xingguang Liu, Juan Liu, Bing Liu, and Xuetao Cao (2018).Phosphorylation-Mediated IFN-γR2 Membrane Translocation Is Required to Activate Macrophage Innate Response.cell.doi.org/10.1016/j.cell.2018.09.011.
      2. Drelich A et al (2018).Exchange Protein Directly Activated by cAMP Modulates Ebola Virus Uptake into Vascular Endothelial Cells. Viruses. doi: 10.3390/v10100563.

      3.Zhu, Y., Shao, F., Yan, W., Xu, Q., & Sun, Y. (2020). Inhibition of SHP2 ameliorates psoriasis by decreasing TLR7 endosome localization. medRxiv.

      4.Thapa, N., Chen, M., Horn, H. T., Choi, S., Wen, T., & Anderson, R. A. (2020). Phosphatidylinositol 3-kinase signalling is spatially organized at endosomal compartments by microtubule-associated protein 4. Nature Cell Biology, 22(11), 1357-1370.

      5.Liang, X. H., Nichols, J. G., Hsu, C. W., & Crooke, S. T. (2020). Hsc70 Facilitates Mannose-6-Phosphate Receptor-Mediated Intracellular Trafficking and Enhances Endosomal Release of Phosphorothioate-Modified Antisense Oligonucleotides. nucleic acid therapeutics.

      6.Jin, J., Kim, C., Xia, Q., Gould, T. M., Cao, W., Zhang, H., ... & Goronzy, J. J. (2021). Activation of mTORC1 at late endosomes misdirects T cell fate decision in older individuals.Science Immunology,6(60).

      7.Gong, B., Guo, Y., Ding, S.et al.A Golgi-derived vesicle potentiates PtdIns4P to PtdIns3P conversion for endosome fission.Nat Cell Biol (2021). https://doi.org/10.1038/s41556-021-00704-y

      8.Huang, F., Tang, X., Ye, B., Wu, S., & Ding, K. (2022). PSL-LCCL: a resource for subcellular protein localization in liver cancer cell line SK_HEP1. Database, 2022.

      9.Ren, X., Zhang, S., Yang, Y., Song, A., Liang, F., Zhang, Y., ... & Xie, Z. (2022). Ketamine induces delirium-like behavior and interferes with endosomal tau trafficking.

      10.Wang, C. K., Nelepcu, I., Hui, D., Oo, H. Z., Truong, S., Zhao, S., ... & Daugaard, M. (2022). Internalization and trafficking of CSPG-bound recombinant VAR2CSA lectins in cancer cells. Scientific Reports, 12(1), 1-13.

      11.Zhu, Y., Wu, Z., Yan, W., Shao, F., Ke, B., Jiang, X., ... & Sun, Y. (2022). Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis. EMBO molecular medicine, 14(3), e14455.

      12.Weishu Ren, Haoyang Lou, Xinghua Ren, Gehua Wen, Xue Wu, Xi Xia, Shuying Wang, Xiaojin Yu, Lei Yan, Guohua Zhang, Jun Yao, Yan Lu, Xu Wu,Ketamine promotes the amyloidogenic pathway by regulating endosomal pH,Toxicology,Volume 471,
      2022

      13.Peipei Ding, Yanqing Xu, Luying Li, Xinyue Lv, Ling Li, Jianfeng Chen, Danlei Zhou, Xiaochao Wang, Qi Wang, Wei Zhang, Tian Liao, Qing-Hai Ji, Qun-Ying Lei, Weiguo Hu(2022).Intracellular complement C5a/C5aR1 stabilizes β-catenin to promote colorectal tumorigenesis,Cell Reports,Volume 39, Issue 9.

      14.Lee, J., Mohammad, N., Lu, Y., Kang, K., Han, K., & Brantly, M. (2022). Alu RNA induces NLRP3 expression through TLR7 activation in α-1-antitrypsin–deficient macrophages. JCI Insight, 7(12).





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