我们的产品-亚细胞结构及组分分离
返回

作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术（北京）有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品，其中具有多个特色产品，为蛋白提取分 离带来了新方案， 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中，产品 以其稳定的品质获得了世界各国越来越多的科研，药物开发及临床诊 断机构的青睐。



    订购信息：ER-036 / 5831.00元(20 Preps)

    Minute™ 动物细胞/组织内质网富集试剂盒 ER-036

    Minute™ ER Enrichment Kit For Tissues and Cultured Cells

    描述：

    内质网是连接核膜和质膜功能的主要膜结构。内质网在真核细胞蛋白转运的胞外途径中起重要作用。在细胞质中合成的蛋白质被运送到内质网，囊泡将蛋白质运送到高尔基体，然后与质膜融合。传统分离内质网的方法是基于密度梯度超离心，需要大量的起始样品，方法繁琐，耗时，交叉污染严重。目前，所有内质网分离的商用试剂盒都是基于上世纪70年代开发的方法。与市场上的内质网分离试剂盒不同，我们的内质网分离试剂盒使用离心管柱技术，简单、快速，只需少量的起始培养细胞或组织，不需要使用杜恩斯匀浆管和超高速离心，就能从培养的细胞/组织中分离出天然ER(主要是粗面内质网)。整个操作可以在大约2h内完成。


    储存温度：

    4℃


    产品视频
    参考文献：ER-036文献列表

    • 1.Zhu, Y., Shao, F., Yan, W., Xu, Q., & Sun, Y. (2020). Inhibition of SHP2 ameliorates psoriasis by decreasing TLR7 endosome localization. medRxiv.

      2.Dowdy, T., Zhang, L., Celiku, O., Movva, S., Lita, A., Ruiz-Rodado, V., ... & Larion, M. (2020). Sphingolipid Pathway as a Source of Vulnerability in IDH1mut Glioma. Cancers, 12(10), 2910.

      3.Lita, A., Pliss, A., Kuzmin, A., Yamasaki, T., Zhang, L., Dowdy, T., ... & Larion, M. (2021). IDH1 mutations induce organelle defects via dysregulated phospholipids. Nature Communications, 12(1), 1-16.

      4.Zhang, L., Li, R., Geng, R., Wang, L., Chen, X. X., Qiao, S., & Zhang, G. (2022). Tumor Susceptibility Gene 101 (TSG101) Contributes to Virion Formation of Porcine Reproductive and Respiratory Syndrome Virus via Interaction with the Nucleocapsid (N) Protein along with the Early Secretory Pathway. Journal of Virology, jvi-00005.

      5.Huang, F., Tang, X., Ye, B., Wu, S., & Ding, K. (2022). PSL-LCCL: a resource for subcellular protein localization in liver cancer cell line SK_HEP1. Database, 2022.

      6.Mondal, T., Shivange, G., Habieb, A., & Tushir-Singh, J. (2022). A Feasible Alternative Strategy Targeting Furin Disrupts SARS-CoV-2 Infection Cycle. Microbiology Spectrum, 10(1), e02364-21.

      7.Zhu, Y., Wu, Z., Yan, W., Shao, F., Ke, B., Jiang, X., ... & Sun, Y. (2022). Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis. EMBO molecular medicine, 14(3), e14455.

      8.Wang, Y., Cao, X., Liu, P., Zeng, W., Peng, R., Shi, Q., ... & Wang, H. (2022). KCTD7 mutations impair the trafficking of lysosomal enzymes through CLN5 accumulation to cause neuronal ceroid lipofuscinoses. Science Advances, 8(31), eabm5578.

      9.Ruiz-Rodado, V., Lita, A., & Larion, M. (2022). Advances in measuring cancer cell metabolism with subcellular resolution. Nature Methods, 1-16.

      10.Ghosh, D. K., Udupa, P., Shrikondawar, A. N., Bhavani, G. S., Shah, H., Ranjan, A., & Girisha, K. M. (2022). Mutant MESD links cellular stress to type I collagen aggregation in osteogenesis imperfecta type XX. Matrix Biology.

      11.Chiu, C. C., Weng, Y. H., Yeh, T. H., Lu, J. C., Chen, W. S., Li, A. H. R., ... & Wang, H. L. (2023). Deficiency of RAB39B Activates ER Stress-Induced Pro-apoptotic Pathway and Causes Mitochondrial Dysfunction and Oxidative Stress in Dopaminergic Neurons by Impairing Autophagy and Upregulating α-Synuclein. Molecular Neurobiology, 1-23.

      12.Edwards-Hicks, J., Apostolova, P., Buescher, J. M., Maib, H., Stanczak, M. A., Corrado, M., ... & Pearce, E. L. (2023). Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function. Nature Immunology, 1-15.

      13.Liu, Y. Y., Bai, J. S., Liu, C. C., Zhou, J. F., Chen, J., Cheng, Y., & Zhou, B. (2023). The Small GTPase Rab14 Regulates the Trafficking of Ceramide from Endoplasmic Reticulum to Golgi Apparatus and Facilitates Classical Swine Fever Virus Assembly. Journal of Virology, e00364-23.

      14.Xiao, X., Shi, J., He, C., Bu, X., Sun, Y., Gao, M., ... & Zhang, J. (2023). ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy. Nature Communications, 14(1), 2859.

      15.Li, Y., Li, K., Pan, T., Xie, Q., Cheng, Y., Wu, X., ... & Cui, S. (2023). Translocation of IGF-1R in endoplasmic reticulum enhances SERCA2 activity to trigger Ca2+ ER perturbation in hepatocellular carcinoma. Acta Pharmaceutica Sinica B.

      16.Zhu, Y., Lei, L., Wang, X., Jiang, Q., Loor, J. J., Kong, F., ... & Li, X. (2023). Low abundance of insulin-induced gene 1 contributes to SREBP-1c processing and hepatic steatosis in dairy cows with severe fatty liver. Journal of Dairy Science.

      17.Zhou, Q., Yang, Y., Xu, Z., Deng, K., Zhang, Z., Hao, J., ... & Li, Y. P. (2023). ATAD1 inhibits hepatitis C virus infection by removing the viral TA‐protein NS5B from mitochondria. EMBO reports, e56614.

      18.Wang, W., Tan, J., Liu, X., Guo, W., Li, M., Liu, X., ... & Zhou, Q. (2023). Cytoplasmic Endonuclease G promotes nonalcoholic fatty liver disease via mTORC2-AKT-ACLY and endoplasmic reticulum stress. Nature Communications, 14(1), 6201.

      19.Guo, C. G., Sun, R., Wang, X., Yuan, Y., Xu, Y., Li, S., ... & Zhang, X. (2023). Intestinal SURF4 is essential for apolipoprotein transport and lipoprotein secretion. Molecular Metabolism, 101847.

      20.Xiong, F. R., Lu, J., Zhu, J. J., Zhao, R. X., Zhang, Y. C., & Yang, J. K. (2024). KCNH6 is essential for insulin secretion by regulating intracellular ER Ca2+ store. The FASEB Journal, 38(4), e23490.

      21.Bi, Y. Y., Chen, Q., Yang, M. Y., Xing, L., & Jiang, H. L. (2024). Nanoparticles targeting mutant p53 overcome chemoresistance and tumor recurrence in non-small cell lung cancer. Nature Communications, 15(1), 1-18.

      22.Bansal, R., Torres, M., Hunt, M., Wang, N., Chatzopoulou, M., Manchanda, M., ... & Wikstrom, J. D. (2024). Role of the mitochondrial protein cyclophilin D in skin wound healing and collagen. JCI insight.




    close
    close