作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术(北京)有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品,其中具有多个特色产品,为蛋白提取分 离带来了新方案, 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中,产品 以其稳定的品质获得了世界各国越来越多的科研,药物开发及临床诊 断机构的青睐。



    订购信息:MP-007 / 5266.00元(50Tests)   MP-007 / 535.00元 (4Tests)

    Minute™ 哺乳动物细胞/组织线粒体分离试剂盒 MP-007

    Minute™ Mitochondrial Isolation Kit for Mammalian Cells and Tissues

    描述:

    哺乳动物细胞/组织线粒体提取试剂盒可以快速的从哺乳动物细胞/组织中提取天然有活性的完整线粒体。离心管柱技术使用方便简单,高产,与其他商业试剂盒相比,可以样品处理范围更广泛,可处理5-50million/样品,缓冲液系统不含表面活性剂和EDTA,可保持线粒体活性。无需匀浆和组织研磨,操作时间<30分钟。

    原理:

    细胞/组织通过Buffer A致敏,然后细胞以Z字形路径通过离心管柱,在这个过程中细胞膜会破裂。进而通过差速离心和密度离心分离出完整的线粒体,整个过程无需匀浆和超高速离心。

    应用:

    提取出的线粒体可以应用于SDS-PAGE,immunoblottings,ELISA,IP,膜蛋白结构分析,2-D,酶活性检测,也可作为提取线粒体DNA,RNA等分子生物学研究的起始原料及其他应用

    储存条件:

    储存于-20℃

    参考文献:MP-007文献列表
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